My research interest is using untargeted metabolomics approach and metabolic flux analysis to study metabolite abundance changes metabolic flux changes during stress responses in Arabidopsis thaliana and Neurospora crassa. Metabolomics is a rapidly growing field of post-genomic biology to identify and quantify the metabolites, the final product in the omics cascade. Untargeted metabolomics analysis focuses on pattern-based classification of as many as possible the metabolites in the biological system with unbiased, global screening. Metabolic flux analysis can measure the metabolic flux, the flow of metabolite through an organism’s metabolic pathway. Applying untargeted metabolomics approach and metabolic flux analysis together can better understand stress-induced metabolic changes in both abundance and flux. My goal is to use these two approaches to study Arabidopsis thaliana abiotic stress responses and Neurospora crassa heat shock stress responses.
Xu Y; Freund DM; Plesofsky N; Brockman S; Brambl R; Hegeman AD; Cohen JD (2014) Untargeted metabolomics of Neurospora crassa wild type and the os-2 mutant under heat shock stress and 2-deoxyglucose treatment, Am. Soc. Mass Spec. 254, Baltimore, MD, (poster).